Application | PCR and primer extension reactions |
---|---|
Blunt-end ligation | |
Precise amplification of bacterial sequences | |
Mutation research |
PfuDNA Polymerase Ultra Pure is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90,000 daltons as estimated from the predicted amino acid sequence and exhibits 3´→5´ exonuclease (proofreading) activity.
PfuDNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity.
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