Synonym | α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride |
---|
PMSF is a serine protease inhibitor that irreversibly inhibits serine proteases by sulfonylation of the serine residue in the active site of the protease. It is widely used in protocols for the isolation of proteins.
PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C. The half-life of a 20mM aqueous solution of PMSF is ~35 minutes at pH 8.0. This short half-life means that aqueous solutions of PMSF can be safely discarded after they have been rendered alkaline (pH >8.6) and stored for several hours at room temperature.
GenDEPOT是一家领先的生物技术公司,致力于开发最有价值的生物研究产品。产品涵盖了生物学研究领域的各项创新技术。GenDEPOT的战略重点放在帮助研究人员改进他们的研究成果,降低成本,节省时间来完成他们的研究目标。此外,他们将通过不断改进我们的产品和服务,以满足我们的所有客户的需要。GenDEPOT产品包括细胞培养、细胞因子、生物检测试剂盒、核酸纯化、蛋白分离与分析、免疫印迹与免疫沉淀。