Appearance | White to off-white powder |
---|---|
Enzymatic Activity | > 40,000 FIP U/mg1 FIP unit of lysozyme is the amount of Enzyme that hydrolyzes under the standard conditions 1 mmole of astandard peptidoglycan per minute. |
Water Content | ≤ 8.0% |
Sulfated Ash | ≤2.0% |
Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between theN-acetylmuramic acid and theN-acetyl-D-glucosamine residues in peptidoglycan.
Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence ofEDTA.
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