Specificity/Cross Reactivity | Tested by gel diffusion and ELISA techniques as applicable. This product reacts specifically with chicken IgY and may recognize other immuno-globulin types that have light chains in common with IgG. Reactivity to IgG subclasses has not been tested. Antibodies to chicken IgY may cross-react with immunoglobulins of other avian species if common binding sites are shared. |
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Suggested Working Concentration | Optimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution. |
ELISA | 1:500 - 1:2500 (1.0 μg/mL - 0.2 μg/mL) |
Western Blot | 1:2500 - 1:10000 (0.2 μg/mL - 0.05 μg/mL) |
Histo/Cytochemical | 1:100 - 1:250 (5.0 μg/mL - 2.0 μg/mL) |
Application | ELISA, Blotting, Histo/Cytochemical |
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