Pore Size | Both WestPure PVDF and NitroPure nitrocellulose membranes are commonly available in two different pore sizes, 0.22 and 0.45 um. Membranes with a pore size of 0.22 um are generally recommended for poteins with a molecular weight of less than 15 kDa, while WestPure PVDF 0.45 um membranes are suitable for most Western blotting applications(5 kDa - 400 kDa protein). |
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Membrane Integrity | PVDF membranes are less fragile than nitrocellulose and generally a better choice for experiments that require stripping and re-probing of the membrane. |
Detection Method | WestPure PVDF and NitroPure Nitrocellulose are both well suited for chemiluminescence and fluorescence detection methods, however WestPure PVDF must be used for fluorescence detection to avoid high background resulting from auto fluorescence of standard PVDF membranes. It is recommended that you cut a small sample of membrane and image it both wet and dry, to check for autofluorescence and background. |
Transfer Buffer | Transfer buffer must contain methanol when using nitrocellulose membranes. Although WestPure PVDF membranes must be pre-wetted with methanol, they can be used with methanol-free transfer buffer. |
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