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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Gendepot/5M Betaine Solution, PCR Certified , B2016/10x1 ml/B2016-010
产品编号:B2016-010
市  场 价:¥1900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$95.00
品      牌: Gendepot
公      司:Gendepot
公司分类:
Gendepot/5M Betaine Solution, PCR Certified , B2016/10x1 ml/B2016-010
商品介绍
Optimal Concentration0.8-1.6M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions
ApplicationPCR

The performance of Betaine Enhancer Solution is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents because of the following effects: Betaine binds preferentially to AT rich sequences in the major groove, thereby stabilizing AT rich regions of the DNA. Because AT forms 2 hydrogen bonds and GC forms 3, the bonding of AT is less stable than the one of GC. As a consequence of the stabilizing effect of Betaine on AT bonding, the stability of AT bonding and GC bonding is brought close to an equal level.

At the same time, Betaine has a sequence independent destabilizing effect on all DNA. The Tm of AT rich and GC rich sequences are equalized and the overall Tm is lowered.

Furthermore, Betaine aids the process of thermostable polymerases and reduces pauses in polymerization caused by secondary structure that can induce the polymerase to disassociate from the DNA strand.

品牌介绍

GenDEPOT是一家领先的生物技术公司,致力于开发最有价值的生物研究产品。产品涵盖了生物学研究领域的各项创新技术。GenDEPOT的战略重点放在帮助研究人员改进他们的研究成果,降低成本,节省时间来完成他们的研究目标。此外,他们将通过不断改进我们的产品和服务,以满足我们的所有客户的需要。GenDEPOT产品包括细胞培养、细胞因子、生物检测试剂盒、核酸纯化、蛋白分离与分析、免疫印迹与免疫沉淀。


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