XpernaseisahighlyacitiveendonucleasefromSerratiamarcescens.ThisenzymeiswidelyusedinbiopharmaceuticalmanufacturingtoquantitativelyeliminatehostcellDNAandRNAduringtheproductionofBIOLOGicalsandvaccines,aswellasforviscosityreductioninbiotechnologyapplications.XpernaseisageneticallyengineeredendonucleaseproducedinEscherichiacoliwithoutinclusionbodyformation.
XpernaseactsasanendonucleasethatdegradesbothDNAandRNA-whethersingle-stranded,double-stranded,linear,circularorsupercoiled.Nobasepairpreferenceisobserved.Aswithallendonucleases,Xpernasehydrolyzesinternalphosphodiesterbondspresentbetweenthenucleotides,Uponcompletedigestion,allfreenucleicacidspresentinsolutionarereducedto5’-monophosphate-terminatedoligonucleotides,whichare2-5basesinlength.